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Gather supplies: bacteriostatic water (BAC water, containing 0.9% benzyl alcohol), insulin syringes (29 to 31 gauge), and alcohol swabs

Three layer gradients of 13%, 16 and 18% (w/v) iodixanol (OptiPrep diluted with HBSS, supplemented with 0.2% BSA (10 ml, 10 ml and 5 ml respectively) are overlaid with the non-parenchymal cells (see Application Sheet C24) in 11% iodixanol and centrifuged at 6,500 g for 30 min [54]

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Table 1 ncRNA regulates tumor Gln metabolism