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Voltage-gated currents were elicited in response to voltage steps from 80 mV to +60 mV in 10 mV increments (V hold = 60mV) using an Axopatch 1D patch clamp amplifier and pCLAMP 11 software (Axon Instruments)

512 Overexpression of Cirbp in the elderly donor heart and supplementation of cardiac arrest fluid with Cirbp agonists reversed the effects of hypothermia