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Women with balanced diets containing animal products typically obtain sufficient B12 from food alone

It also appears to exert a protective effect on the gut-brain axis, reducing symptoms of anxiety and stress-induced damage through modulation of serotonin and dopamine systems

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Fibroblast growth factor 23 impairs phosphorus and vitamin D metabolism in vivo and suppresses 25-hydroxyvitamin D-1alpha-hydroxylase expression in vitro

Laboratory assessment typically involves measuring serum 25(OH)D, though interpretation requires understanding assay variability and population-specific reference ranges