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Our in vitro IGF1-binding assays showed that IGF1R WT and 163GS bound to IGF1 with similar affinities, indicating that the L1 hairpin mutations did not affect IGF1 binding (Fig

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The antibody in the aforementioned IFN- reagent kit was used directly (utilizing the pre-prepared formulation provided with the kit, which required no additional dilution), while the antibody in the TNF- reagent kits was used at a 1:100 dilution

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