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For the reporter assay, cells were transfected with a Gaussia luciferase (GLUC) plasmid containing either the human SIRT1 promoter (HRPM35661-PG02, Genecopoeia, USA) or the mouse HK2 promoter (MPRM42494-PG02, Genecopoeia) sequence, along with a Renilla luciferase plasmid

and Vilardell, J., "Regulated pre-mRNA splicing: the ghostwriter of the eukaryotic genome", Biochem

Sterile water mixes must be discarded within 24 hours, whereas bacteriostatic water preserves the solution for multi-draw use

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