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Food intake and water consumption were measured over 8 consecutive days as in ( 2.4 Serum analysis Blood was collected by cardiac puncture in SST Amber tubes (BD Microtainer ) from five-hour fasted mice

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SOCS proteins bind JAKs or their receptors and act by (i) abolishing the kinase ability of JAK, (ii) abolishing the ability of JAK to bind to the receptor, (iii) blocking STATs from being recruited to the receptor, and (iv) mediating the ubiquitination of JAKs and STATs for degradation (124)