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Hindlimb ischemia models Male mice aged 8 weeks and weighing 2228 g were used in the experiment

Tissue H 2 S measurement The left ventricular homogenates were mixed with 2 mM pyridoxal 50-phosphate, 10 mM l-cysteine and 250 mL 10% trichloroacetic acid to reach a total volume of 750 mL and incubated at 37 C for 30 min

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The numbers were ran, broke ties with annual out-of-pocket cost, and cross-checked each score with fresh patient feedback