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Despite being a simple and direct principle, it remains a key assay due to both the sensitivity and specificity of the detection which emphasizes the importance of the development and optimization of the reaction conditions for various applications
pp 520 Parvin R, Pande SV
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Glutathione, often referred to as the body's "master antioxidant," is crucial for maintaining cellular integrity and supporting various bodily functions
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The reduction of Compound I back to its resting state can occur via the transfer of a hydrogen atom, involving either a His-mediated (FitaRossmann) mechanism where a distal histidine acts as an acidbase catalyst or a direct mechanism that allows for a hydrogen atom transfer
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