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Materials needed Your Wolverine Blend (BPC-157, TB-500) vial (lyophilized) Alcohol swabs Bacteriostatic sterile water 3 mL syringes (Luer Lock tip) 25G or 27G needles (Luer Lock)

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Here, we benchmarked several engineered laccase templates 22,23,24,25 , identified an ancestral fungal variant with slight activity in HEK293T cells and performed extensive directed evolution on the yeast surface to improve its activity (Fig

14 , e1002472 (2016)