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Many bacteria, such as Borrelia burgdorferi (the Lyme Disease organism),yeasts and some viruses, are susceptible to high levels of oxygen in their immediate cellular environment

There are three possibilities for the differential response of BPA on esr1 and esr2 in our retinal homogenates: (1) absence of ER in retinal tissue, (2) differential affinity for ER and ER by BPA, and (3) posttranslational modification of activated ER

The caveat is an open bottle is only good for 28 days after opening

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At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS