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Quantitative real-time PCR (qRT-PCR) of mRNA and microbial DNA Total RNA from tissues was extracted using TRIzol reagent (Yamei, Shanghai, China) and reverse-transcribed into complementary DNA (cDNA) with a reverse transcription kit (Vazyme, Nanjing, China)

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Gently roll or swirl the vial between your fingers until the powder fully dissolves

GCGR: the negative control Glucagon is on the chart only to be ruled out