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The hydrophobicity of the phenylalanine cluster in crevasse, along with the ability of fatty acids longer than octanoic acid (C8:0), such as lauric acid (C12:0) and palmitic acid (C16:0), to activate the ghrelin receptor, initially led to the hypothesis that the fatty acid chain of ghrelin extends through this crevasse into the cell membrane ( Figure 3 Structural insights into ghrelin recognition by the ghrelin receptor Following the publication of the inactive ghrelin receptor structure in 2020, a series of ghrelin receptor structures were determined using cryo-EM and subsequently reported (Qin et al., 2022

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25% (w/v) Nycodenz, 10 mM EDTA was diluted with Hanks Balanced Salt Solution (HBBS) (containing 10 mM EDTA) to produce two solutions of approx