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Samples were obtained at sequential time points after the addition of 2-deoxyglucose (2-DG) and diamide

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Fabs were obtained by papain digestion of anti-PV1 MAbs and then purified using a HiTrap Capto DEAE column (Cytiva)

The fewer samples in 2021 were mainly due to the COVID-19 pandemic in 2020, as samples that reached the laboratory were usually seized much earlier

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Calcium, calcification, and melatonin biosynthesis in the human pineal gland: a postmortem study into age-related factors