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Single-cell differential gene expression analysis between hepatocytes from semaglutide-versus vehicle-treated animals revealed a strong upregulation of genes involved in amino acid biosynthesis and metabolism, a trend shared across nearly all hepatic cell types ( Figure 6D-E )

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Number and Brightness (N&B) analysis of the RICCS cross-correlation data was then used to determine the effect of the V229A substitution on receptor oligomerisation before and after exendin-4 stimulation (Figure 5A)

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