Vascular permeability factor/vascular endothelial growth factor and the significance of microvascular hyperpermeabiltiy in angiogenesis
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Shen, D
Handbook of Experimental Pharmacology, vol 104 / 1
After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader
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