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Cells were grown to roughly 85% confluence and CSE was diluted in the media to a final concentration of 20%

Cloudiness, sedimentation, or rapid degradation appear within hours or days, and the user has no way to tell whether the formula was wrong (it wasn't) or the bac water was bad (it was)

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But, being careful is key, mainly for those at high risk. Vitamin B12 is key for making myelin, the fatty stuff around nerve fibers

doi:10.1073/pnas.84.8.2377

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