Subsequently, primary antibodies were used for overnight incubation under 4C: NLRP3 (1:1,000), GSDMD (1:1,000), NF-B p65 (1:1,000), p-NF-B p65 (1:1,000), IL-1 (1:1,000), IL-18 (1:1,000), ASC (1:1,000), Caspase-1 (1:1,000), Cleaved-Caspase-1 (1:1,000), PI3K (1:1,000), p-PI3K (1:1,000), AKT (1:1,000), p-AKT (1:1,000), IB- (1:1,000), -actin (1:10,000), and GAPDH (1:10,000)
PEVs loaded with MCC950 (an NLRP3 inflammasome inhibitor) reduced atherosclerotic plaque formation and localized vascular inflammation [89,90,91,92], highlighting PEVs as biocompatible, autologous drug carriers [93]
[MDPI] 41
With the rising prevalence of diabetes, understanding its effects on male reproductive function and subsequent generations has become increasingly important
The Journal of Physical Chemistry B 107 , 11941207, doi:10.1021/jp026827s (2003)
PubMed, Geraedts, Maartje C