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After an additional twenty-four hours, cells were removed from the incubator, placed on ice, lysed (without a PBS wash) with 1 mL of ice cold 80% methanol/20% water solution containing 1.5 M metabolomics amino acid mix (Cambridge Isotope Laboratories, MSK-A2-1.2)

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Directions for use: Must be administered under physician supervision

Baldini G, Phelan KD

Residual Solvents Determined in accordance with the general method for the determination of residual solvents (General Chapters 0861 Method 2)