After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples
Kashin-Beck disease Kashin-Beck disease (KBD) is an endemic condition that affects an estimated 2.5 million people in Tibet, northern and central China, North Korea, and southeastern Siberia, a region of Russia (58)
We hope to propose a dual strategy of iron metabolism regulation and inflammation inhibition, breaking through the traditional single-target limitations
When administered properly under medical supervision, serious adverse effects are rare
Abais-Battad JM, Dasinger JH, Fehrenbach DJ, Mattson DL
Cell Chem Biol (2019) 26(3):42032.e9