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The typical gradient was 10% to 40% component B over 30 minutes at a flow rate of 1 ml/min at 37C where component A was 80 mM triethy amine phosphate, pH 3.0 and component B was acetonitrile (ACN)

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Currently, there is no crystal structure available for the JAK2 pseudokinase-kinase to allow observation of the interaction interface between each domain

H.ChengH.XuFengR.YuanY.et al (2012)