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In mutant BRAF melanoma cells, although inhibition of BRAF V600E by vemurafenib reduced HIF-1 protein, TXNIP and ARRDC4 were shown to be upregulated by binding of the alternative transcription factor MondoA to the promoters of these genes [70]

Other endoscopic approaches target the stomach: One in common use today applies sutures to fold the stomach and shrink its size, while another, more experimental method burns off stomach tissue that regulates the secretion of appetite-stimulating hormones

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Cells were treated in normal media at 37 C with vehicle, 100 nM exendin-4 for 5 minutes or 100 nM GIP for the indicated times, washed once with ice cold PBS and lysed with 1 mL lysis buffer supplemented with 1% phosphatase inhibitor cocktail (Sigma-Aldrich) and 1% complete EDTA-free protease inhibitor cocktail (SigmaAldrich) on ice

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