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doi: 10.1016/j.pharmthera.2006.11.007 52 BrandT

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We then formulated the following MILP to integrate additional reactions required for the core reactions to carry flux: where m is the number of metabolites in the network, n is the number of reactions in the network, nc is the number of non-core reactions, z k are binary variables for all the non-core reactions, S is the stoichiometric matrix, v are the net fluxes for all the reactions and are the corresponding net-forward and net-reverse fluxes, so that, v i = , for all i = 1,, n