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The membranes were blocked with 5% nonfat dry milk in PBS supplemented with 0.1% (v/v) Tween 20 and incubated overnight at 4C with anti-CPT2, PPAR (Santa Cruz Biotechnology,United States), CPT1A, LCAD, Very long-chain acyl-CoA dehydrogenase (VLCAD), (Proteintech, United States), GAPDH (from Cell Signaling Technology, United States)

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Injections can raise blood levels quickly and relieve deficiency symptoms, but they are not a validated weight-loss therapy for healthy adults