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The protein extracts were separated by 12% SDS-polyacrylamide gel electrophoresis and then electroblotted onto polyvinylidene difluoride membranes

Fruits, leafy greens, and water were the only categories that saw a net increase in consumption

While moderate weight reduction is often therapeutically beneficialparticularly in improving metabolic disorders such as type 2 diabetes and obesitysubstantial or prolonged weight loss has been increasingly implicated in adverse skeletal outcomes

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Cellular migration and interaction models