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Tirzepatide is the older, fully matured story: a single-molecule dual incretin agonist taken from discovery through Phase 1 proof-of-concept, [7] then through the large SURPASS (diabetes) and SURMOUNT (obesity) Phase 3 programs, and finally to FDA and EMA approval

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Biochemical determination of antioxidant enzymes Glutathione peroxidase (GPx, EC 1.11.1.9) activity was determined using BioVision tests (BioVision, Mountain View, CA, USA)

GH secretion parameters, including basal secretion, pulse frequency (number of peaks per 12 h), pulse area, and half-life, were determined using the automated deconvolution algorithm AutoDecon (23), which has been well-validated for deconvolution analysis of endogenous GH pulsatility