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10.1001/jama.2013.2107 151 LeeJ.-S.SongI.-H.ShindeP

Assembly of the L-Carnitine Biosynthetic Pathway After efficient enzymes for the last and penultimate step of the L-carnitine biosynthetic pathway had been identified, enzymes for the first two steps were selected: TML hydroxylase and an HTML aldolase (Supplementary Table 1)

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To potentially increase access for the antibodies, we used isolated cell envelopes for our labeling experiments, but again failed to observe labeling of the PilQ nozzles at cell-cell junctions

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There are, however, a few limitations in this study