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Allegretti JR, Kassam Z, Mullish BH, Chiang A, Carrellas M, Hurtado J, et al

In brief, serial dilutions of G49 and Fc-GLP1 in assay buffer (as above) were added in duplicate to CHO cells recombinantly expressing human GLP-1R and incubated for 30 min at RT before detection with the cAMP dynamic 2 HTRF assay